Title: As-tolerant P-A strain of Pantoea ananatis: a potential PGPR, isolated from the Hezhou mining area in China
Microbial and plant assisted bioremediation is an emerging way for the remediation of soils polluted with heavy metals. To screen the arsenic tolerant bacteria, soil samples were collected from Nanjing mining area, China. The average cadmium content of the mine soil reached 45.71 mg/kg, which was indicating serious pollution and potential ecological risk. From the mine soil, six arsenic tolerant plant growth promoting rhizobacteria (PGPR) were isolated. The isolated bacterial strain “P-A” showed maximum arsenic tolerance and it was selected for further experimentation. This strain was identified as Pantoea ananatis by 16S rRNA gene sequencing. P-A was found to tolerate maximum arsenic at 2.0 mM concentration. This strain also exhibited good adsorption capacity (up to 45.7%) of heavy metal at 1 mM concentration. Results of this study exhibited organic phosphorus solubilization (37.08 mg/L) and IAA biosynthesis (18.11 mg/L) ability of isolated P. ananatis. Scanning electron microscopy (SEM) revealed cell shrinkage and the cell wall of P. ananatis became rough at 1 mM arsenic stress. FT-IR study described the differences between functional groups and nature of chemical bonds between and after the absorption of arsenic by P. ananatis. At 0.25 mM arsenic concentration, P. ananatis treated seeds of Capsicum annuum L. developed 2.46 times longer roots than untreated seeds. Results of this study helped us to conclude that P-A strain of P. ananatis possesses significant metal tolerance and bioremediation potential against arsenic. In future, this strain can be used as a microbial remediation agent to detoxify heavy metals in contaminated soils.
Fiza Liaquat has done her PhD in Shanghai Jiao tong University. During her PhD she published 7 research articles as a first author in reputed journals She also published one patent and developed very good collaboration with other labs and published 6 research articles with Dr. Jianxin Shi of School of Life Science SJTU, Dr. Yidong Zhang of School of Plant Science SJTU, and Dr. M. Farooq Hussain Munis, Quaid-i-Azam University, Islamabad, Pakistan. She also participated and presented her research work in high profile conferences in Chenshan Botanical Garden, Shanghai Forestry Station, Tongji University and Shanghai International Flower Show. She Got the 1st prize in the Shanghai Botany Youth Paper Conference. She Represented SJTU, China as “invited student speaker” in International Conference entitled “Building innovative Pakistan” in Quaid-i-Azam University, Islamabad.
Title: The Sustainable Management of I dioscopus clypealis (Mango Leaf Hopper) at Ratnagiri District, India.
India is very famous for ‘Alphonso Mango' in the international market. However, due to indiscriminate use of agrochemicals, growth regulators, and fertilizers, reduces the exportable quality of fruit. Therefore, it is well felt need to adopt sustainable pest management. In the cursory survey, it is found that Idioscopus clypealis (Mango leafhopper) is the major pest in the study area.The present study aimed towards the development of bio-insecticide and to check its potential against Mango leafhopper. Strychnos nux vomica extract contains alkaloids (Strychnine and Brucine) which are responsible for the insecticidal property. The efficacy of Srtychnos nux vomica extract against mango leafhopper was checked in three different solvents (viz. Petroleum ether, Ethanol, Ethyl acetate) and the value obtained was statistically significant i.e. p<0.05. From the phytotoxicity test, it is concluded that a high amount of Neem oil is not good for plant health. The liquid insecticide was formulated in the form of emulsifiable concentrates (EC). Different concentrations are prepared and all four (A1, A2, A3, A4) concentrations of the formulation are further analyzed for the bioassay for 24 hrs. The bioassay results showed that the formulation 'A1' and 'A2' showed 100% mortality after 24 hrs.However, ‘A3’ and ‘A4’ showed comparatively less mortality. However ‘A1’and ‘A2’ have comparatively more amounts of Neem oil and it showing 100%mortality and it shows phytotoxicity. Hence, Formulation 'A3" and 'A4' are considered for further analysis. The results obtained from bioassay it is very clear that formulation 'A4' showed less mortality than 'A3' with no phytotoxic effect.
Dr. Tari Vinaya Satyawan Savitri is currently working as an “External Consultant” at GauEcoGram Agrovikas Producer Company Private Limited, Pune, Maharashtra, India. She has completed her Ph.D. in Environmental Science from the University of Mumbai. She did under graduation studies in Zoology, M.Sc. in Environmental Science, and a Diploma in Biotechnology (2 years) from the University of Mumbai and B.Ed. from S.N.D.T. University. She has qualified State Level Eligibility Test for Assistant Professorship valid in the states of Maharashtra and Goa, India. She has been awarded the 'Young Scientist Award' from Deccan Environmental Research Organization (DERO). She has attended around 33 conferences/ workshops/ webinars/ faculty development programme. She has published more than 20 research items in reputed journals, Magazines/e-Newsletters, and conferences.