Title: NEW OFF-LINE SPE-GC-MS/MS METHOD FOR DETERMINATION OF MOSH/MOAH IN ANIMAL FEED, FOODS, INFANT FORMULA AND VEGETABLE OILS

Abstract:

MOH (mineral oil hydrocarbons) which consist of mineral oil saturated

hydrocarbons(MOSH) and mineral oil aromatic hydrocarbons(MOAH) are present in various

products such as vegetable oils, animal feed, foods and infant formula. Contamination of foods with

mineral oil hydrocarbons, particularly mineral oil aromatic hydrocarbons(MOAH),exhibiting

carcinogenic, mutagenic and hormone-disruptive effects. Identifying toxic substances among the many

thousands comprising mineral oils in food samples is a difficult analytical challenge. A new method

based on an offline-solid phase extraction approach coupled with gas chromatography-triple

quadrupole(GC-MS/MS) was developed for determination of MOSH/MOAH in various products such

as vegetable oils, animal feed, foods and infant formula. A glass SPE cartridge loaded with 7 g of

activated silica gel impregnated with 10 % silver nitrate for removal of olefins and lipids. The

MOSH/MOAH fractions in the oil samples were eluated with hexane and hexane: dichloromethane

:toluene(75:20:5), respectively. Each eluate was concentrated to 50 µl in toluene and injected on

splitless mode into GC-MS/MS. Accuracy of the method was estimated as measurement of recovery of

spiked oil samples at 2.0, 15.0 and 30.0 mg kg -1 , and recoveries varied from 85 to 105 %. The method

was applied to the different types of samples (sunflower meal, chocolate ships, santa milk chocolate,

biscuits, infant milk, cornflakes, refined sunflower oil, crude sunflower oil), detecting MOSH up to 56

mg/kg and MOAH up to 5 mg/kg. The limit of quantification(LOQ) of proposed method was estimated

at 0.5 mg/kg and 0.3 mg/kg for MOSH and MOAH ,respectively.

Biography:

Ovanes Chakoyan studied  Organic Synthesis at the University of Chemical Technology and Metallurgy of Sofia, Bulgaria as Bachelor in 2008. He is chief researcher/chromatography expert in Fidelitas Lab, Bulgaria.He received his  M.D. of Organic Chemistry from University of Shumen in 2022. He has over 10 years practical skills in areas of gas chromatography and liquid chromatography, including developing new analytical methods and sample preparation in environment, soil, water, air pollution and food matrices.